Synaptopodin is required for long-term depression at Schaffer collateral-CA1 synapses.

Publisher: BioMed Central Country of Publication: England NLM ID: 101468876 Publication Model: Electronic Cited Medium: Internet ISSN: 1756-6606 (Electronic) Linking ISSN: 17566606 NLM ISO Abbreviation: Mol Brain Subsets: MEDLINE

Original Publication: London : BioMed Central

Depression* ; Schaffer Collaterals*; Animals ; Mice ; Hippocampus/metabolism ; Long-Term Potentiation/physiology ; Long-Term Synaptic Depression/physiology ; Neuronal Plasticity/physiology ; Synapses/metabolism

Synaptopodin (SP), an actin-associated protein found in telencephalic neurons, affects activity-dependant synaptic plasticity and dynamic changes of dendritic spines. While being required for long-term depression (LTD) mediated by metabotropic glutamate receptor (mGluR-LTD), little is known about its role in other forms of LTD induced by low frequency stimulation (LFS-LTD) or spike-timing dependent plasticity (STDP). Using electrophysiology in ex vivo hippocampal slices from SP-deficient mice (SPKO), we show that absence of SP is associated with a deficit of LTD at Sc-CA1 synapses induced by LFS-LTD and STDP. As LTD is known to require AMPA- receptors internalization and IP3-receptors calcium signaling, we tested by western blotting and immunochemistry if there were changes in their expression which we found to be reduced. While we were not able to induce LTD, long-term potentiation (LTP), albeit diminished in SPKO, can be recovered by using a stronger stimulation protocol. In SPKO we found no differences in NMDAR, which are the primary site of calcium signalling to induce LTP. Our study shows, for the first time, the key role of the requirement of SP to allow induction of activity-dependant LTD at Sc-CA1 synapses.
(© 2024. The Author(s).)

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MOP 86724 Canada CAPMC CIHR

Keywords: IP3; LTD; STDP; Synaptic plasticity; Synaptopodin; hippocampus

0 (Synpo protein, mouse)

Date Created: 20240402 Date Completed: 20240404 Latest Revision: 20240405

20240405

PMC10988887

10.1186/s13041-024-01089-3

38566234