Primary Culture of Dissociated Neurons from the Embryonic Cerebral Cortex.

Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE

Publication: Totowa, NJ : Humana Press
Original Publication: Clifton, N.J. : Humana Press,

Neurons* ; Axons*; Cerebral Cortex

Primary neuronal culture is a valuable in vitro model for analyzing the molecular mechanisms underlying the development and function of neural circuits. In contrast to neurons in vivo, primary cultured neurons can easily be transfected with genes of interest or treated with chemicals such as agonists and inhibitors of a specific target molecule. Furthermore, time-dependent morphological changes, such as the acquisition of neuronal polarity, axon elongation, and dendrite branch formation, can be analyzed by using primary neuronal cultures. Here, we describe a method for preparing a primary culture of neurons from the developing cerebral cortex, together with a method for gene transfer to primary cultured cortical neurons.
(© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)

Dotti CG, Sullivan CA, Banker GA (1988) The establishment of polarity by hippocampal neurons in culture. J Neurosci 8(4):1454–1468. (PMID: 10.1523/JNEUROSCI.08-04-01454.198832820386569279)
Brachet A, Lario A, Fernández-Rodrigo A, Heisler FF, Gutiérrez Y, Lobo C, Kneussel M, Esteban JA (2021) A kinesin 1-protrudin complex mediates AMPA receptor synaptic removal during long-term depression. Cell Rep 36(5):109499. https://doi.org/10.1016/j.celrep.2021.109499. (PMID: 10.1016/j.celrep.2021.10949934348158)
Grabrucker A, Vaida B, Bockmann J, Boeckers TM (2009) Synaptogenesis of hippocampal neurons in primary cell culture. Cell Tissue Res 338(3):333–341. https://doi.org/10.1007/s00441-009-0881-z. (PMID: 10.1007/s00441-009-0881-z19885679)
Shikanai M, Nishimura YV, Sakurai M, Nabeshima YI, Yuzaki M, Kawauchi T (2018) Caveolin-1 promotes early neuronal maturation via caveolae-independent trafficking of N-cadherin and L1. iScience 7:53–67. https://doi.org/10.1016/j.isci.2018.08.014. (PMID: 10.1016/j.isci.2018.08.014302676866135901)
Kawauchi T, Chihama K, Nabeshima Y, Hoshino M (2003) The in vivo roles of STEF/Tiam1, Rac1 and JNK in cortical neuronal migration. EMBO J 22(16):4190–4201. (PMID: 10.1093/emboj/cdg41312912917175802)
Kawauchi T, Chihama K, Nabeshima Y, Hoshino M (2006) Cdk5 phosphorylates and stabilizes p27kip1 contributing to actin organization and cortical neuronal migration. Nat Cell Biol 8(1):17–26. (PMID: 10.1038/ncb133816341208)
Shikanai M, Ito S, Nishimura YV, Akagawa R, Fukuda M, Yuzaki M, Nabeshima YI, Kawauchi T (2023) Rab21 regulates caveolin-1-mediated endocytic trafficking to promote immature neurite pruning. EMBO Rep 24(3):e54701. https://doi.org/10.15252/embr.202254701. (PMID: 10.15252/embr.202254701366835679986827)

Keywords: Cerebral cortex; Dissociated cell culture; Embryonic brain; Mouse; Neuron; Primary culture

Date Created: 20240417 Date Completed: 20240418 Latest Revision: 20240418

20240418

10.1007/978-1-0716-3810-1_14

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