Quantifying genome-specific carbon fixation in a 750-meter deep subsurface hydrothermal microbial community.

Publisher: Oxford University Press Country of Publication: England NLM ID: 8901229 Publication Model: Print Cited Medium: Internet ISSN: 1574-6941 (Electronic) Linking ISSN: 01686496 NLM ISO Abbreviation: FEMS Microbiol Ecol Subsets: MEDLINE

Publication: 2015- : Oxford Oxford University Press
Original Publication: [Amsterdam] : Elsevier Science Publishers on behalf of the Federation of European Microbiological Societies, [1985-

Carbon Cycle* ; Bicarbonates*/metabolism ; Microbiota* ; Carbon Isotopes*/metabolism ; Metagenome*; Bacteria/genetics ; Bacteria/metabolism ; Bacteria/classification ; Carbon/metabolism ; Hydrothermal Vents/microbiology ; Groundwater/microbiology ; Chemoautotrophic Growth ; Archaea/genetics ; Archaea/metabolism

Dissolved inorganic carbon has been hypothesized to stimulate microbial chemoautotrophic activity as a biological sink in the carbon cycle of deep subsurface environments. Here, we tested this hypothesis using quantitative DNA stable isotope probing of metagenome-assembled genomes (MAGs) at multiple 13C-labeled bicarbonate concentrations in hydrothermal fluids from a 750-m deep subsurface aquifer in the Biga Peninsula (Turkey). The diversity of microbial populations assimilating 13C-labeled bicarbonate was significantly different at higher bicarbonate concentrations, and could be linked to four separate carbon-fixation pathways encoded within 13C-labeled MAGs. Microbial populations encoding the Calvin-Benson-Bassham cycle had the highest contribution to carbon fixation across all bicarbonate concentrations tested, spanning 1-10 mM. However, out of all the active carbon-fixation pathways detected, MAGs affiliated with the phylum Aquificae encoding the reverse tricarboxylic acid (rTCA) pathway were the only microbial populations that exhibited an increased 13C-bicarbonate assimilation under increasing bicarbonate concentrations. Our study provides the first experimental data supporting predictions that increased bicarbonate concentrations may promote chemoautotrophy via the rTCA cycle and its biological sink for deep subsurface inorganic carbon.
(© The Author(s) 2024. Published by Oxford University Press on behalf of FEMS.)

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364653263 Deutsche Forschungsgemeinschaft; GO 3267/2-1 DFG

Keywords: DNA stable isotope probing; deep biosphere; microbial carbon fixation; qSIP

0 (Bicarbonates)
0 (Carbon Isotopes)
7440-44-0 (Carbon)

Date Created: 20240417 Date Completed: 20240507 Latest Revision: 20240509

20240509

PMC11075769

10.1093/femsec/fiae062

38632042