Evaluation optimum ratio of synthetic bone graft material and platelet rich fibrin mixture in a metal 3D printed implant to enhance bone regeneration.

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  • Author(s): Wong KW;Wong KW; Chen YS; Chen YS; Lin CL; Lin CL
  • Source:
    Journal of orthopaedic surgery and research [J Orthop Surg Res] 2024 May 16; Vol. 19 (1), pp. 299. Date of Electronic Publication: 2024 May 16.
  • Publication Type:
    Journal Article
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: BioMed Central Country of Publication: England NLM ID: 101265112 Publication Model: Electronic Cited Medium: Internet ISSN: 1749-799X (Electronic) Linking ISSN: 1749799X NLM ISO Abbreviation: J Orthop Surg Res Subsets: MEDLINE
    • Publication Information:
      Original Publication: London : BioMed Central, 2006-
    • Subject Terms:
      Printing, Three-Dimensional* ; Platelet-Rich Fibrin* ; Bone Regeneration*/drug effects ; Bone Regeneration*/physiology ; Titanium*; Animals ; Rabbits ; Swine ; Femur/surgery ; Bone Substitutes ; Bone Transplantation/methods ; Prostheses and Implants
    • Abstract:
      Background: This study aims to evaluate the optimal ratio of synthetic bone graft (SBG) material and platelet rich fibrin (PRF) mixed in a metal 3D-printed implant to enhance bone regeneration.
      Methods: Specialized titanium hollow implants (5 mm in diameter and 6 mm in height for rabbit; 6 mm in diameter and 5 mm in height for pig) were designed and manufactured using 3D printing technology. The implants were divided into three groups and filled with different bone graft combinations, namely (1) SBG alone; (2) PRF to SBG in 1:1 ratio; (3) PRF to SBG in 2:1 ratio. These three groups were replicated tightly into each bone defect in distal femurs of rabbits (nine implants, n = 3) and femoral shafts of pigs (fifteen implants, n = 5). Animal tissue sections were obtained after euthanasia at the 8th postoperative week. The rabbit specimens were stained with analine blue, while the pig specimens were stained with Masson-Goldner's trichrome stain to perform histologically examination. All titanium hollow implants were well anchored, except in fracture specimens (three in the rabbit and one fracture in the pig).
      Result: Rabbit specimens under analine blue staining showed that collagen tissue increased by about 20% and 40% in the 1:1 ratio group and the 2:1 ratio group, respectively. Masson-Goldner's trichrome stain results showed that new bone growth increased by 32% in the 1:1 ratio PRF to SBG, while - 8% in the 2:1 ratio group.
      Conclusion: This study demonstrated that placing a 1:1 ratio combination of PRF and SBG in a stabilized titanium 3D printed implant resulted in an optimal increase in bone growth.
      (© 2024. The Author(s).)
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    • Contributed Indexing:
      Keywords: 3D printing; Bone defect; Bone regeneration; Platelet rich fibrin; Synthetic bone graft
    • Publication Date:
      Date Created: 20240516 Date Completed: 20240517 Latest Revision: 20240519
    • Publication Date:
      20240519
    • Accession Number:
      PMC11097440
    • Accession Number:
      10.1186/s13018-024-04784-y
    • Accession Number:
      38755635