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Edisto Library
Closed for renovations
Phone: (843) 869-2355
Main Library
9 a.m. - 5 p.m.
Phone: (843) 805-6930
West Ashley Library
9 a.m. - 5 p.m.
Phone: (843) 766-6635
Folly Beach Library
Closed for renovations
Phone: (843) 588-2001
John L. Dart Library
9 a.m. - 5 p.m.
Phone: (843) 722-7550
St. Paul's/Hollywood Library
9 a.m. - 5 p.m.
Phone: (843) 889-3300
Mt. Pleasant Library
9 a.m. – 5 p.m.
Phone: (843) 849-6161
Dorchester Road Library
9 a.m. - 5 p.m.
Phone: (843) 552-6466
Edgar Allan Poe/Sullivan's Island Library
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John's Island Library
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Phone: (843) 559-1945
McClellanville Library
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Wando Mount Pleasant Library
9 a.m. - 5 p.m.
Phone: (843) 805-6888
Otranto Road Library
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Phone: (843) 572-4094
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9 a.m. - 5 p.m.
Phone: (843) 766-2546
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Phone: (843) 805-6892
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9 a.m. - 1 p.m.
Phone: (843) 884-9741
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Activated caspase expression and apoptosis increase in keloids: cytochrome c release and caspase-9 activation during the apoptosis of keloid fibroblast lines.
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- Author(s): Akasaka, Yoshikiyo (AUTHOR); Ito, Kinji (AUTHOR); Fujita, Kazuko (AUTHOR); Komiyama, Kazuo (AUTHOR); Ono, Ichiro (AUTHOR); Ishikawa, Yukio (AUTHOR); Akishima, Yuri (AUTHOR); Sato, Hiroko (AUTHOR); Ishii, Toshiharu (AUTHOR)
- Source:
Wound Repair & Regeneration. Jul/Aug2005, Vol. 13 Issue 4, p373-382. 10p. - Source:
- Additional Information
- Subject Terms:
- Abstract: To characterize apoptosis in keloids and the mechanisms responsible for this process, the expression of activated caspase-9 and -3 in fibroblasts obtained from keloids was analyzed. Immunohistochemistry revealed that the number of fibroblasts positive for terminal deoxynucleotide transferase-mediated dUTP nick-end labeling (TUNEL) or activated caspase-9 or -3 was low but was significantly higher in keloid tissues than in normal scar tissues. Significant relationships between the number of caspase-positive fibroblasts and TUNEL-positive fibroblasts suggested that the activation of caspase-9 and -3 induces apoptosis in a subpopulation of keloid fibroblasts. All keloid fibroblast cell lines established in this study showed activation of caspase-9 and -3 after serum deprivation for 3 or 4 hours, as shown using Western blotting. Furthermore, serum deprivation-induced apoptosis in a keloid fibroblast line was blocked by a caspase-9 inhibitor (acetyl-Leu-Glu-His-Asp-al), indicating that activation of caspase-9 was necessary for the process of apoptosis in keloid fibroblasts. Although serum deprivation did not significantly change the level of apoptosis protease activating factor-1 in any of the lines, cytochrome c release was detected in cytosolic fractions of the lines after serum deprivation for 3 or 4 hours. These results strongly suggest that keloid fibroblasts are predisposed to apoptosis and cytochrome c release and that caspase-9 activation may underlie regulation of apoptosis in keloid fibroblasts in vivo. [ABSTRACT FROM AUTHOR]
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