The tumor-suppressive microRNA-143/145 cluster inhibits cell migration and invasion by targeting GOLM1 in prostate cancer.

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  • Additional Information
    • Source:
      Publisher: Nature Pub. Group Country of Publication: England NLM ID: 9808008 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1435-232X (Electronic) Linking ISSN: 14345161 NLM ISO Abbreviation: J Hum Genet Subsets: MEDLINE
    • Publication Information:
      Publication: 2009- : London : Nature Pub. Group
      Original Publication: Tokyo : Springer-Verlag, c1998-
    • Subject Terms:
      Cell Movement* ; Gene Expression Regulation* ; Multigene Family*; Membrane Proteins/*biosynthesis ; MicroRNAs/*metabolism ; Neoplasm Proteins/*biosynthesis ; Prostatic Neoplasms/*metabolism ; RNA, Neoplasm/*metabolism; Aged ; Cell Line, Tumor ; Humans ; Male ; Membrane Proteins/genetics ; MicroRNAs/genetics ; Middle Aged ; Neoplasm Invasiveness ; Neoplasm Proteins/genetics ; Prostatic Neoplasms/genetics ; Prostatic Neoplasms/pathology ; RNA, Neoplasm/genetics
    • Abstract:
      Our recent study of microRNA (miRNA) expression signature of prostate cancer (PCa) has revealed that the microRNA-143/145 (miR-143/145) cluster is significantly downregulated in cancer tissues, suggesting that these cluster miRNAs are candidate tumor suppressors. The aim of this study was to investigate the functional significance of the miR-143/145 cluster in PCa cells and to identify novel targets regulated by these cluster miRNAs in PCa. Restoration of miR-143 or miR-145 in PCa cell lines (PC3 and DU145) revealed that these miRNAs significantly inhibited cancer cell migration and invasion. Gene expression data and in silico analysis demonstrated that Golgi membrane protein 1 (GOLM1) resembling a type II golgi transmembrane protein was a potential target of miR-143/145 cluster target gene. Gene expression studies and luciferase reporter assays showed that GOLM1 was directly regulated by the miR-143/145 cluster. Silencing of GOLM1 resulted in significant inhibition of cell migration and invasion in PCa cells. Furthermore, the expression of GOLM1 was upregulated in cancer tissues by immunohistochemistry. Loss of the tumor-suppressive miR-143/145 cluster enhanced cancer cell migration and invasion in PCa through directly regulating GOLM1. Our data on target genes regulated by the tumor-suppressive miR-143/145 cluster provide new insights into the potential mechanisms of PCa oncogenesis and metastasis.
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    • Accession Number:
      0 (GOLM1 protein, human)
      0 (MIRN143 microRNA, human)
      0 (MIRN145 microRNA, human)
      0 (Membrane Proteins)
      0 (MicroRNAs)
      0 (Neoplasm Proteins)
      0 (RNA, Neoplasm)
    • Publication Date:
      Date Created: 20131129 Date Completed: 20140724 Latest Revision: 20220330
    • Publication Date:
      20231215
    • Accession Number:
      10.1038/jhg.2013.121
    • Accession Number:
      24284362