Two Genetically Distinct Plasmodium knowlesi Duffy Binding Protein Alpha Region II (PkDBPαII) Haplotypes Demonstrate Higher Binding Level to Fy(a+b+) Erythrocytes than Fy(a+b--) Erythrocytes.

Publisher: American Society of Tropical Medicine and Hygiene Country of Publication: United States NLM ID: 0370507 Publication Model: Print Cited Medium: Internet ISSN: 1476-1645 (Electronic) Linking ISSN: 00029637 NLM ISO Abbreviation: Am J Trop Med Hyg Subsets: MEDLINE

Publication: Northbrook, IL : American Society of Tropical Medicine and Hygiene
Original Publication: Baltimore.

Duffy Blood-Group System*/immunology; Antigens, Protozoan/*genetics ; Erythrocytes/*parasitology ; Plasmodium knowlesi/*metabolism ; Protozoan Proteins/*genetics ; Receptors, Cell Surface/*genetics; Antigens, Protozoan/immunology ; Antigens, Protozoan/metabolism ; Binding Sites/genetics ; Borneo ; Haplotypes ; Humans ; Malaria/parasitology ; Malaysia ; Plasmodium knowlesi/genetics ; Protozoan Proteins/immunology ; Protozoan Proteins/metabolism ; Receptors, Cell Surface/immunology ; Receptors, Cell Surface/metabolism

Invasion of human erythrocytes by merozoites of Plasmodium knowlesi involves interaction between the P. knowlesi Duffy binding protein alpha region II (PkDBPαII) and Duffy antigen receptor for chemokines (DARCs) on the erythrocytes. Information is scarce on the binding level of PkDBPαII to different Duffy antigens, Fy ^a and Fy ^b . This study aims to measure the binding level of two genetically distinct PkDBPαII haplotypes to Fy(a+b-) and Fy(a+b+) human erythrocytes using erythrocyte-binding assay. The binding level of PkDBPαII of Peninsular Malaysian and Malaysian Borneon haplotypes to erythrocytes was determined by counting the number of rosettes formed in the assay. Overall, the Peninsular Malaysian haplotype displayed higher binding activity than the Malaysian Borneon haplotype. Both haplotypes exhibit the same preference to Fy(a+b+) compared with Fy(a+b-), hence justifying the vital role of Fy ^b in the binding to PkDBPαII. Further studies are needed to investigate the P. knowlesi susceptibility on individuals with different Duffy blood groups.

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0 (Antigens, Protozoan)
0 (Duffy Blood-Group System)
0 (Duffy antigen binding protein, Plasmodium)
0 (Protozoan Proteins)
0 (Receptors, Cell Surface)

Date Created: 20200320 Date Completed: 20200630 Latest Revision: 20210502

20231215

PMC7204561

10.4269/ajtmh.19-0836

32189613