Growth Inhibition and Apoptotic Effect of Pine Extract and Abietic Acid on MCF-7 Breast Cancer Cells via Alteration of Multiple Gene Expressions Using In Vitro Approach.

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  • Additional Information
    • Source:
      Publisher: MDPI Country of Publication: Switzerland NLM ID: 100964009 Publication Model: Electronic Cited Medium: Internet ISSN: 1420-3049 (Electronic) Linking ISSN: 14203049 NLM ISO Abbreviation: Molecules Subsets: MEDLINE
    • Publication Information:
      Original Publication: Basel, Switzerland : MDPI, c1995-
    • Subject Terms:
      Abietanes/*pharmacology ; Antineoplastic Agents, Phytogenic/*pharmacology ; Gene Expression Regulation, Neoplastic/*drug effects ; Pinus/*chemistry ; Plant Extracts/*pharmacology; Abietanes/chemistry ; Antineoplastic Agents, Phytogenic/chemistry ; Antioxidants/chemistry ; Antioxidants/pharmacology ; Apoptosis/drug effects ; Cell Cycle Checkpoints/drug effects ; Cell Proliferation/drug effects ; Dose-Response Relationship, Drug ; Flow Cytometry ; Humans ; Immunohistochemistry ; MCF-7 Cells ; Plant Extracts/chemistry
    • Abstract:
      In vitro anti-proliferative activity of Pinus palustris extract and its purified abietic acid was assessed against different human cancer cell lines (HepG-2, MCF-7 and HCT-116) compared to normal WI-38 cell line. Abietic acid showed more promising IC 50 values against MCF-7 cells than pine extract (0.06 µg/mL and 0.11 µM, respectively), with insignificant cytotoxicity toward normal fibroblast WI-38 cells. Abietic acid triggered both G 2 /M cell arrest and subG 0 -G 1 subpopulation in MCF-7, compared to SubG 0 -G 1 subpopulation arrest only for the extract. It also induced overexpression of key apoptotic genes ( Fas, FasL, Casp3, Casp8, Cyt-C and Bax ) and downregulation of both proliferation ( VEGF, IGFR1, TGF-β ) and oncogenic ( C-myc and NF-κB ) genes. Additionally, abietic acid induced overexpression of cytochrome-C protein. Furthermore, it increased levels of total antioxidants to diminish carcinogenesis and chemotherapy resistance. P. palustris is a valuable source of active abietic acid, an antiproliferative agent to MCF-7 cells through induction of apoptosis with promising future anticancer agency in breast cancer therapy.
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    • Contributed Indexing:
      Keywords: MCF-7; P. palustris; abietic aid; anti-cancer; apoptosis
    • Accession Number:
      0 (Abietanes)
      0 (Antineoplastic Agents, Phytogenic)
      0 (Antioxidants)
      0 (Plant Extracts)
      V3DHX33184 (abietic acid)
    • Publication Date:
      Date Created: 20220111 Date Completed: 20220208 Latest Revision: 20240405
    • Publication Date:
      20240405
    • Accession Number:
      PMC8746537
    • Accession Number:
      10.3390/molecules27010293
    • Accession Number:
      35011526