Genomic Characterization and Safety Assessment of Bifidobacterium breve BS2-PB3 as Functional Food.

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  • Additional Information
    • Source:
      Publisher: Korean Society for Microbiology and Biotechnology Country of Publication: Korea (South) NLM ID: 9431852 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1738-8872 (Electronic) Linking ISSN: 10177825 NLM ISO Abbreviation: J Microbiol Biotechnol Subsets: MEDLINE
    • Publication Information:
      Publication: 2002- : Seoul : Korean Society for Microbiology and Biotechnology
      Original Publication: Seoul : Korean Society for Applied Microbiology, 1991-2001.
    • Subject Terms:
      Probiotics* ; Genome, Bacterial* ; Bifidobacterium breve*/genetics ; Anti-Bacterial Agents*/pharmacology ; Phylogeny* ; Microbial Sensitivity Tests*; Functional Food ; Virulence Factors/genetics ; Whole Genome Sequencing ; Drug Resistance, Bacterial/genetics ; Base Composition ; Humans ; Genomics ; Antioxidants/pharmacology
    • Abstract:
      Our group had isolated Bifidobacterium breve strain BS2-PB3 from human breast milk. In this study, we sequenced the whole genome of B. breve BS2-PB3, and with a focus on its safety profile, various probiotic characteristics (presence of antibiotic resistance genes, virulence factors, and mobile elements) were then determined through bioinformatic analyses. The antibiotic resistance profile of B. breve BS2-PB3 was also evaluated. The whole genome of B. breve BS2-PB3 consisted of 2,268,931 base pairs with a G-C content of 58.89% and 2,108 coding regions. The average nucleotide identity and whole-genome phylogenetic analyses supported the classification of B. breve BS2-PB3. According to our in silico assessment, B. breve BS2-PB3 possesses antioxidant and immunomodulation properties in addition to various genes related to the probiotic properties of heat, cold, and acid stress, bile tolerance, and adhesion. Antibiotic susceptibility was evaluated using the Kirby-Bauer disk-diffusion test, in which the minimum inhibitory concentrations for selected antibiotics were subsequently tested using the Epsilometer test. B. breve BS2-PB3 only exhibited selected resistance phenotypes, i.e. , to mupirocin (minimum inhibitory concentration/MIC >1,024 μg/ml), sulfamethoxazole (MIC >1,024 μg/ml), and oxacillin (MIC >3 μg/ml). The resistance genes against those antibiotics, i.e. , ileS , mupB , sul4 , mecC and ramA , were detected within its genome as well. While no virulence factor was detected, four insertion sequences were identified within the genome but were located away from the identified antibiotic resistance genes. In conclusion, B. breve BS2-PB3 demonstrated a sufficient safety profile, making it a promising candidate for further development as a potential functional food.
    • Contributed Indexing:
      Keywords: Bifidobacterium breve; antibiotic resistance; human breast milk; microbiota; probiotic safety
    • Accession Number:
      0 (Anti-Bacterial Agents)
      0 (Virulence Factors)
      0 (Antioxidants)
    • Publication Date:
      Date Created: 20240318 Date Completed: 20240426 Latest Revision: 20240426
    • Publication Date:
      20240426
    • Accession Number:
      10.4014/jmb.2311.11031
    • Accession Number:
      38494884