Effect of S-region mutations on HBsAg in HBsAg-negative HBV-infected patients.

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  • Author(s): Liu H;Liu H; Chen S; Chen S; Liu X; Liu X; Lou J; Lou J
  • Source:
    Virology journal [Virol J] 2024 Apr 23; Vol. 21 (1), pp. 92. Date of Electronic Publication: 2024 Apr 23.
  • Publication Type:
    Journal Article; Research Support, Non-U.S. Gov't
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: BioMed Central Country of Publication: England NLM ID: 101231645 Publication Model: Electronic Cited Medium: Internet ISSN: 1743-422X (Electronic) Linking ISSN: 1743422X NLM ISO Abbreviation: Virol J Subsets: MEDLINE
    • Publication Information:
      Original Publication: [London] : BioMed Central, 2004-
    • Subject Terms:
      Hepatitis B Surface Antigens*/genetics ; Hepatitis B Surface Antigens*/immunology ; Hepatitis B virus*/genetics ; Hepatitis B virus*/immunology ; Mutation* ; DNA, Viral*/genetics ; Hepatitis B*/virology; Humans ; Female ; Male ; Adult ; Middle Aged ; Amino Acid Substitution ; Genotype ; Young Adult ; Aged
    • Abstract:
      Background: Occult HBV infection (OBI) is a special form of hepatitis B virus (HBV) infection that may cause Liver cirrhosis and hepatocellular carcinoma, causing significant harm to patients. Given the insidious nature of OBI, it is usually not easy to be detected. Most of the samples currently studied are concentrated on blood donors, however, patients in this special state have not been fully studied. This project aimed to study the effect of HBV S region mutations on HBsAg in patients with clinical OBI.
      Methods: Collect 107 HBsAg-/HBV DNA + blood samples from Beijing Youan Hospital, Capital Medical University from August 2022 to April 2023. Next, the successfully extracted and amplified HBV DNA S regions were sequenced. Construct mutant plasmids to verify the cell function of the high-frequency mutation sites and explore the possible molecular mechanism.
      Results: Sixty-eight HBsAg-negative samples were sequenced, revealing high-frequency amino acid substitution sites in the HBV S protein, including immune escape mutations (i.e., sY100C、sK122R、sI126T、sT131P、and sS114T) and TMD (Transmembrane domain) region substitutions (i.e., sT5A、sG10D、sF20S、and sS3N). We constructed a portion of the mutant plasmids and found that sT5A, sF20S, sG10D, sS3N, sI68T, and sI126T single point mutations or combined mutations may decrease HBsAg expression or change the antigenicity of HBsAg leading to detection failure.
      Conclusions: HBsAg-negative patients may show various mutations and amino acid replacement sites at high frequency in the HBV S-region, and these mutations may lead to undetectable Hepatitis B surface antigen (HBsAg), HBsAg antigenic changes or secretion inhibition.
      (© 2024. The Author(s).)
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    • Grant Information:
      BJYAYY-CY2019-11 Beijing Youan Hospital affiliated to Capital Medical University incubates young and middle-aged talents
    • Contributed Indexing:
      Keywords: HBV S region; Hepatitis B Virus; Mutation; Occult HBV infection
    • Accession Number:
      0 (Hepatitis B Surface Antigens)
      0 (DNA, Viral)
    • Publication Date:
      Date Created: 20240423 Date Completed: 20240424 Latest Revision: 20240513
    • Publication Date:
      20240514
    • Accession Number:
      PMC11040738
    • Accession Number:
      10.1186/s12985-024-02366-2
    • Accession Number:
      38654327